Background: An unexpected antibody screening test with panel cells from abroad without Dia positive cells is common practice in most laboratories in Korea, although it is impossible to detect anti-Dia. Our laboratory have used DiaCell Dia (DiaMed Ag, Cressier, Morat, Switzerland) as one of antibody screening panel cells and only positive samples with DiaCell Dia cells have been also confirmed with Dia antigen positive panel cells from Korean Red Cross (KRC). In this study, the experiences and clinical usefulness of DiaCell Dia were evaluated.
Methods: The 26,102 pretransfusion samples were tested by DiaMed microcolumn gel assay using the LISS/Coombs card, ID-DiaCell I, II, and DiaCell Dia. The DiaMed microcolumn gel assay using the LISS/Coombs card has been used for unexpected antibody screening test with panel cells from KRC.
Results: The positive detection rate of unexpected antibody screening test using ID-DiaCell I and II was 0.58%(151/26, 102), and eleven samples were reactive (1+~2+) with DiaCell Dia panel cells (0.04%). However, only six(0.02%) of 11 samples were reactive(2+) with Dia positive cells from KRC.
Conclusion: The discrepant detection rate for unexpected antibody with Dia panel cells between from DiaMed and KRC is supposed to need further study. The addition of Dia positive cells as an unexpected antibody screening panel cells is strongly recommended in case that antiglobulin phase of crossmatch is abbreviated for unimmunized patients.
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